Characterization of the periplasmic region of PomB , a Na + - driven flagellar 1 stator protein in
نویسندگان
چکیده
Characterization of the periplasmic region of PomB, a Na + -driven flagellar 1 stator protein in Vibrio alginolyticus 2 3 4 Na Li 1,2 , Seiji Kojima 2* and Michio Homma 2 5 6 1 Division of Microbiology, Graduate School of Life Science, Northwest A&F 7 University, Yanglin, Shaanxi, Yanglin 712100, China; 2 Division of Biological 8 Science, Graduate School of Science, Nagoya University, Furo-cho, Chikusa-ku, 9 Nagoya 464-8602, Japan 10 11 Running title: The periplasmic region of PomB 12 13
منابع مشابه
Assembly of motor proteins, PomA and PomB, in the Na+-driven stator of the flagellar motor.
PomA and PomB are transmembrane proteins that form the stator complex in the sodium-driven flagellar motor of Vibrio alginolyticus and are believed to surround the rotor part of the flagellar motor. We constructed and observed green fluorescent protein (GFP) fusions of the stator proteins PomA and PomB in living cells to clarify how stator proteins are assembled and installed into the flagellar...
متن کاملSodium-dependent dynamic assembly of membrane complexes in sodium-driven flagellar motors.
The bacterial flagellar motor is driven by the electrochemical potential of specific ions, H(+) or Na(+). The motor consists of a rotor and stator, and their interaction generates rotation. The stator, which is composed of PomA and PomB in the Na(+) motor of Vibrio alginolyticus, is thought to be a torque generator converting the energy of ion flux into mechanical power. We found that specific ...
متن کاملCell-free synthesis of the torque-generating membrane proteins, PomA and PomB, of the Na+-driven flagellar motor in Vibrio alginolyticus.
Flagellar motor proteins, PomA and PomB, are essential for converting the sodium motive force into rotational energy in the Na(+)-driven flagella motor of Vibrio alginolyticus. PomA and PomB, which are cytoplasmic membrane proteins, together comprise the stator complex of the motor and form a Na(+) channel. We tried to synthesize PomA and PomB by using the cell-free protein synthesis system, PU...
متن کاملCharacterization of PomA mutants defective in the functional assembly of the Na(+)-driven flagellar motor in Vibrio alginolyticus.
The polar flagellar motor of Vibrio alginolyticus rotates using Na(+) influx through the stator, which is composed of 2 subunits, PomA and PomB. About a dozen stators dynamically assemble around the rotor, depending on the Na(+) concentration in the surrounding environment. The motor torque is generated by the interaction between the cytoplasmic domain of PomA and the C-terminal region of FliG,...
متن کاملDeletion analysis of the carboxyl-terminal region of the PomB component of the vibrio alginolyticus polar flagellar motor.
The stator of the sodium-driven flagellar motor of Vibrio alginolyticus is a membrane protein complex composed of four PomA and two PomB subunits. PomB has a peptidoglycan-binding motif in the C-terminal region. In this study, four kinds of PomB deletions in the C terminus were constructed. None of the deletion proteins restored motility of the DeltapomB strain. The PomA protein was coisolated ...
متن کامل